Antibacterial Surfaces

2 October 2018

DTU Lyngby
Meeting room 1
Anker Engelundsvej 1
2800 Kgs. Lyngby

Bacteria affect all aspects of our daily life – although often undetected. However, it can have catastrophic consequences if not controlled. Bacterial adhesion and biofilm formation on material surfaces represent a serious problem in society from both an economical and health perspective. Effective antibacterial surfaces that prevent bacterial adhesion and growth are of increasing importance due to the increasing prevalence of antibiotic resistance bacterial strains.

The aim of this one-day symposium is to gain a better understanding of bacterial growth from planktonic cells to the protective life in biofilm structures, which completely changes the way we look at bacteria but also the efficiency of the remedies we have at hand today. Many strategies exist for preventing bacterial contamination and spread from a surface, and based on different surface technologies new approaches to antibacterial surfaces will be presented during the symposium. When developing new antibacterial surfaces, it is of outmost importance to document its efficiency and safe use.

During the last part of the symposium, we will hear about how antibacterial surfaces are to be tested and what requirements are needed for bringing a new antibacterial surface to the Danish and European market, i.e. what tests should be applied to the product and how this is evaluated by the regulatory organs (EU/DK).

09:30 - 10:00 Registration
10:00 - 10:10 Welcome
10:10 - 10:40
Bacterial adhesion and biofilm: a challenge in clinical settings and processing of food and pharma products

Lone Gram, Professor, DTU

Bacteria and other microorganisms affect all aspects of our life. They are key players in the biogeochemical cycles and provide nutrients for growth of higher organisms. They are also a major challenge as infectious agents of man, animals and plants. Until a few decades ago, most studies of bacteria were done in liquid mono-cultures where the organisms grew as planktonic cells. However, it was realized that the dominant mode of microbial growth is as adhered cells on biotic or abiotic surfaces, so-called biofilms. These consist of the microbes, water and polymeric substances (proteins, DNA, polysaccharides). Microorganisms in biofilms are often more tolerant to antimicrobials such as antibiotics and disinfectants and, hence can constitute a continuous reservoir of contamination and infection. Thus, preventing or reducing microbial adhesion to surfaces is key in infection control and in food and pharma processing. However, microbial biofilms may also be beneficial and therefore enhancing biofilm formation is important in for instance biological sewage treatment or in plant biocontrol where beneficial bacteria colonize the roots and offer plant protection.

Lone Gram, PhD, is the leader of the Center for Microbial Secondary Metabolites, one of 10 Centers of Excellence funded by the Danish National Research Foundation. She became professor in bacteriology in 2000 and has published more than 200 peer reviewed articles. She received the Villum Annual Award in 2016.
10:40 - 11:10
Investigation of Chronic Biofilm Infection

Kasper Nørskov Kragh, Postdoctoral Fellow, University of Copenhagen

Structured, surface-attached biofilms are rarely observed in chronic infections. Rather, chronic biofilm infections constitute a highly complex environment where non-attached, aggregated biofilms are heterogeneously distributed within highly inflamed mucus or pus. Foreign body related infections are an exception to this and bacterial biofilms have been observed surrounding pacemaker wires, stents and catheters. In these instances, biofilm may build up in the interface between the foreign body and host tissue, though often to a lesser extent directly on the surface itself. In this talk, I will give an overview of the current understanding reguarding biofilm in chronic infections, both unattached and surface bound. Additionally, I will compare and contrast one of the most widely used in vitro biofilm models with relevant in vivo findings.

Kasper Nørskov Kragh is Post Doc. from The Costerton Biofilm Center where he investigates how biofilm aggregates are involvement in pathogenesis. He has been closely involved in the development of new innovative in vitro infection models, which as closely possible resembles the environmental conditions we can observe in the chronic infection themselves as well.
11:10 - 11:30 Break
11:30 - 12:00
Biofilm on a surface: diagnosis and treatment

Trine Rolighed Thomsen, Head of Section, Biotech, Life Science, Danish Technological Institute

Biofilms are of great importance in infection control and healthcare-associated infections. Biofilms develop on medical device surfaces and several strategies are being developed and tested to minimize the risk. Selected projects focusing on prevention, diagnosis and treatment of surface related infections will be presented showing data from in vitro and in vivo systems.

Trine Rolighed Thomsen heads a research group on medical biotechnology at University of Aalborg and the Danish Technological Institute. Investigations of microbial abundance, diversity, distribution and function in various infections aiming for optimized diagnosis and treatment is a core area of the group.
12:00 - 12:30
Intelligent Biocides

Christopher James Lüscher, CEO, reACT.Global

Infectious control is facing more and more challenges with the development of resistance in microbes. Traditional biocides and methods are having difficulty handling the growing global threat from listeria in food to hospital acquired infections (HAI). These microbes cost society increasing amounts. Therefore, this allows for a new view on infectious control using state of the art technology for this new class of biocides.

Christopher James Lüscher is a technologically driven manager, who has been working with nano-technology for more than a decade. He co-developed his first commercial nano product in the early 2000’s. In the last 5 years, he has focused on creating better hygiene solutions and services by developing state of the art technologies.
12:30 - 13:30 Lunch
13:30 - 14:00
An antibacterial copper-silver alloy as potential strategy for reducing spread of pathogenic agents in health-care settings

Nicole Ciacotich, Industrial PhD fellow at Elplatek A/S and DTU Bioengineering, DTU

The emergence and spread of antibiotic resistance and multidrug-resistant pathogenic bacteria is an increasing challenge in health care settings. Bacteria may spread via inert surfaces and therefore antimicrobial materials are being developed as a potential strategy for reducing the contamination of frequently touched surfaces such as door handles and furniture in hospitals. Her project has demonstrated that a novel electroplated copper-silver alloy on stainless steel has higher antimicrobial activity than the individual metals in standardized adhesion tests, suggesting it as a promising tool for controlling the spread of pathogens in hospitals. The direct contact between the alloy surface and bacteria induces a significant pH increase, which could play a key role in the antibacterial mechanism. The talk will describe the copper-silver coating and its ways of action.

Nicole Ciacotich is an industrial PhD student at Elplatek A/S and DTU Bioengineering in the Bacterial Ecophysiology and Biotechnology group. She collaborates with the Dept. of Immunology and Microbiology at Copenhagen University and the Dept. of Mechanical Engineering at DTU.
14:00 - 14:30
The role of surface properties in bacterial adhesion and biofilm development

Guanghong Zeng, Scientist, DFM (Danish National Metrology Institute)

Fighting bacteria and biofilm related infections has proven to be a challenging effort which requires multiplex approaches. Reducing bacterial adhesion through surface modification is an attractive tool due to the low toxicity and low risk of inducing resistance. To achieve this, we need to better understand how surface properties affect bacterial adhesion, and develop tools to study and evaluate adhesion of bacteria and growth of biofilm. In this talk, Guanghong Zeng is going to talk about using atomic force microscopy to study adhesion of bacterial cells on various surface, and its applications in studying the role of bacterial surface molecules, and in evaluating antifouling coatings.

Guanghong Zeng is a Scientist at the Danish National Metrology Institute where he is currently working on surface characterization with scanning probe microscopy, optical imaging, and vibrational spectroscopy.
14:30 - 14:50 Break
14:50 - 15:20
Methods to study bacterial biofilm

Karen Angeliki Krogfelt, Professor, Statens Serum Institut

Studies of microbial pathogenesis and drug development require the use of bot in vitro an in vivo methodology. Methods are presented here, that are used in order to understand bacterial binding capacity and the molecular mechanisms behind it that result in infection.

Karen A. Krogfelt, Statens Serum Institut, works on diagnosis and pathogenesis of bacterial infections.
15:20 - 15:50
Controlling Variation of Microbiological Methods – experience from industry (PEF)

Morten Alhede, Managing Partner, Emendo R&D

Medical devices: Novel technologies enable development of advanced antimicrobial medical devices such as coated surfaces and materials. In spite of few written requirements, FDA expects that test methods used to produce data in support of regulatory filings are validated. Microbiological methods are no exception, but these methods are widely accepted as being extremely variable and hence difficult to validate. In this talk, considerations, pitfalls and other experiences from designing microbiological test methods in support of antimicrobial technologies are discussed.

Morten Alhede, PhD, is Managing Partner at Emendo R&D and does consulting for pharma and medtech companies.
15:50 - 16:00 Closing remarks

Registration fee
Members of ATV-SEMAPP or other promoting organizations: DKK 2,150
Non-members: DKK 2,775
M.Sc. students: DKK 200 & Ph.D. students: DKK 975 (Membership is free of charge. Early bird discount does not apply)
Early bird discount of DKK 200 when registering before 15 September 2018.

All prices are excluded of Danish VAT.

The fee includes talks, breakfast, lunch and coffee breaks.

Cancellation

Cancellations received before 24 September 2018 are fully refunded. No refund for cancellations received on 24 September or later, nor for being unable to attend on the day.Substitutions are accepted at any time.

Questions

Please do not hesitate to contact ATV-SEMAPP by e-mailing semapp@atv-semapp.dk or phoning Jytte Laursen +45 4525 4898 or Charlotte Leser +45 4525 4899.